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OCR: Ives The heterogeneous assay technique inv er the separation of bound and free reactants a The immunological reaction has taken place separation can be in liquid or solid phas In the liquid-phase separation procedur tibody, is body that isprecipitating agent, usually a second anti rated by directed against the hormone-binding a used. The precipitate thus formed is sep centrifugation. rm is the Less time-consuming and easier to perfc que, either solid-phase separation. With this techni olid the antibody or the antigen is fixed on a he reaction support; this support can be the wall of t separation vessel or a solid particle. The solid-phas technique is the most widely used.